[HTML][HTML] Delayed phosphorylation of classical protein kinase C (PKC) substrates requires PKC internalization and formation of the pericentrion in a phospholipase D …
MA El-Osta, J Idkowiak-Baldys, YA Hannun - Journal of Biological …, 2011 - ASBMB
It was previously demonstrated that sustained activation (30–60 min) of protein kinase C
(PKC) results in translocation of PKC α and βII to the pericentrion, a dynamic subset of the
recycling compartment whose formation is dependent on PKC and phospholipase D (PLD).
Here we investigated whether the formation of the pericentrion modulates the ability of PKC
to phosphorylate substrates, especially if it reduces substrate phosphorylation by
sequestering PKC. Surprisingly, using an antibody that detects phosphosubstrates of …
(PKC) results in translocation of PKC α and βII to the pericentrion, a dynamic subset of the
recycling compartment whose formation is dependent on PKC and phospholipase D (PLD).
Here we investigated whether the formation of the pericentrion modulates the ability of PKC
to phosphorylate substrates, especially if it reduces substrate phosphorylation by
sequestering PKC. Surprisingly, using an antibody that detects phosphosubstrates of …