[HTML][HTML] Role of zymogen and activated factor X as scaffolds for the inhibition of the blood coagulation factor VIIa-tissue factor complex by recombinant nematode …
PW Bergum, A Cruikshank, SL Maki, CR Kelly… - Journal of Biological …, 2001 - ASBMB
Recombinant nematode anticoagulant protein c2 (rNAPc2) is a potent, factor Xa (fXa)-
dependent small protein inhibitor of factor VIIa-tissue factor (fVIIa· TF), which binds to a site
on fXa that is distinct from the catalytic center (exo-site). In the present study, the role of other
fX derivatives in presenting rNAPc2 to fVIIa· TF is investigated. Catalytically active and active
site blocked fXa, as well as a plasma-derived and an activation-resistant mutant of zymogen
fX bound to rNAPc2 with comparable affinities (KD= 1–10 nm), and similarly supported the …
dependent small protein inhibitor of factor VIIa-tissue factor (fVIIa· TF), which binds to a site
on fXa that is distinct from the catalytic center (exo-site). In the present study, the role of other
fX derivatives in presenting rNAPc2 to fVIIa· TF is investigated. Catalytically active and active
site blocked fXa, as well as a plasma-derived and an activation-resistant mutant of zymogen
fX bound to rNAPc2 with comparable affinities (KD= 1–10 nm), and similarly supported the …