[HTML][HTML] Accurate quantification of lipid species affected by isobaric overlap in Fourier-transform mass spectrometry
M Höring, CS Ejsing, S Krautbauer, VM Ertl… - Journal of Lipid …, 2021 - ASBMB
Lipidomics data require consideration of ions with near-identical masses, which comprises
among others the Type-II isotopic overlap. This overlap occurs in series of lipid species
differing only by number of double bonds (DBs) mainly because of the natural abundance of
13 C-atoms. High-resolution mass spectrometry, such as Fourier-transform mass
spectrometry (FTMS), is capable of resolving Type-II overlap depending on mass resolving
power. In this work, we evaluated FTMS quantification accuracy of lipid species affected by …
among others the Type-II isotopic overlap. This overlap occurs in series of lipid species
differing only by number of double bonds (DBs) mainly because of the natural abundance of
13 C-atoms. High-resolution mass spectrometry, such as Fourier-transform mass
spectrometry (FTMS), is capable of resolving Type-II overlap depending on mass resolving
power. In this work, we evaluated FTMS quantification accuracy of lipid species affected by …