Acute renal failure during sepsis: potential role of cell cycle regulation
Q Yang, D Liu, Y Long, H Liu, W Chai, XT Wang - Journal of Infection, 2009 - Elsevier
OBJECTIVES: This study aimed to evaluate cell cycle regulation in acute kidney injury after
intraperitoneal sepsis in rats. METHODS: Polymicrobial sepsis was induced by cecal ligation
and puncture (CLP) in rats. At 0, 6, 12, 24, 48, and 72h after CLP, serum creatinine was
evaluated. DNA content of isolated kidney cells was analyzed using flow cytometer.
Furthermore, the expression of p21, p53, cyclin D1, cyclin E, CDK2, CDK4 and P-pRb was
also measured by western blot. RESULTS: After sepsis-induced by CLP, kidney injury of rat …
intraperitoneal sepsis in rats. METHODS: Polymicrobial sepsis was induced by cecal ligation
and puncture (CLP) in rats. At 0, 6, 12, 24, 48, and 72h after CLP, serum creatinine was
evaluated. DNA content of isolated kidney cells was analyzed using flow cytometer.
Furthermore, the expression of p21, p53, cyclin D1, cyclin E, CDK2, CDK4 and P-pRb was
also measured by western blot. RESULTS: After sepsis-induced by CLP, kidney injury of rat …