[HTML][HTML] Furin-cleaved proprotein convertase subtilisin/kexin type 9 (PCSK9) is active and modulates low density lipoprotein receptor and serum cholesterol levels
MT Lipari, W Li, P Moran, M Kong-Beltran, T Sai… - Journal of Biological …, 2012 - ASBMB
Proprotein convertase subtilisin/kexin 9 (PCSK9) regulates plasma LDL cholesterol levels
by regulating the degradation of LDL receptors. Another proprotein convertase, furin,
cleaves PCSK9 at Arg 218-Gln 219 in the surface-exposed" 218 loop." This cleaved form
circulates in blood along with the intact form, albeit at lower concentrations. To gain a better
understanding of how cleavage affects PCSK9 function, we produced recombinant furin-
cleaved PCSK9 using antibody Ab-3D5, which binds the intact but not the cleaved 218 loop …
by regulating the degradation of LDL receptors. Another proprotein convertase, furin,
cleaves PCSK9 at Arg 218-Gln 219 in the surface-exposed" 218 loop." This cleaved form
circulates in blood along with the intact form, albeit at lower concentrations. To gain a better
understanding of how cleavage affects PCSK9 function, we produced recombinant furin-
cleaved PCSK9 using antibody Ab-3D5, which binds the intact but not the cleaved 218 loop …