Simultaneous analysis of cortisol and cortisone in saliva using XLC–MS/MS for fully automated online solid phase extraction
RL Jones, LJ Owen, JE Adaway, BG Keevil - Journal of Chromatography B, 2012 - Elsevier
Salivary cortisol measurements are increasingly being used in the investigation of disorders
of the hypothalamic–pituitary–adrenal axis. In the salivary gland, cortisol is metabolised to
cortisone by the action of 11β-hydroxysteroid dehydrogenase type 2, and cortisone is partly
responsible for the variable interference observed in current salivary cortisol immunoassays.
The aim of this study was to validate an assay for the simultaneous analysis of salivary
cortisol and cortisone using the Spark Holland Symbiosis™ in eXtraction liquid …
of the hypothalamic–pituitary–adrenal axis. In the salivary gland, cortisol is metabolised to
cortisone by the action of 11β-hydroxysteroid dehydrogenase type 2, and cortisone is partly
responsible for the variable interference observed in current salivary cortisol immunoassays.
The aim of this study was to validate an assay for the simultaneous analysis of salivary
cortisol and cortisone using the Spark Holland Symbiosis™ in eXtraction liquid …