Circulating microRNA profiles in human patients with acetaminophen hepatotoxicity or ischemic hepatitis

J Ward, C Kanchagar… - Proceedings of the …, 2014 - National Acad Sciences
J Ward, C Kanchagar, I Veksler-Lublinsky, RC Lee, MR McGill, H Jaeschke, SC Curry
Proceedings of the National Academy of Sciences, 2014National Acad Sciences
We have identified, by quantitative real-time PCR, hundreds of miRNAs that are dramatically
elevated in the plasma or serum of acetaminophen (APAP) overdose patients. Most of these
circulating microRNAs decrease toward normal levels during treatment with N-acetyl
cysteine (NAC). We identified a set of 11 miRNAs whose profiles and dynamics in the
circulation during NAC treatment can discriminate APAP hepatotoxicity from ischemic
hepatitis. The elevation of certain miRNAs can precede the dramatic rise in the standard …
We have identified, by quantitative real-time PCR, hundreds of miRNAs that are dramatically elevated in the plasma or serum of acetaminophen (APAP) overdose patients. Most of these circulating microRNAs decrease toward normal levels during treatment with N-acetyl cysteine (NAC). We identified a set of 11 miRNAs whose profiles and dynamics in the circulation during NAC treatment can discriminate APAP hepatotoxicity from ischemic hepatitis. The elevation of certain miRNAs can precede the dramatic rise in the standard biomarker, alanine aminotransferase (ALT), and these miRNAs also respond more rapidly than ALT to successful treatment. Our results suggest that miRNAs can serve as sensitive diagnostic and prognostic clinical tools for severe liver injury and could be useful for monitoring drug-induced liver injury during drug discovery.
National Acad Sciences