A quick method for the isolation of glomeruli from human kidney.

AH Nagi, W Kirkwood - Journal of clinical pathology, 1972 - ncbi.nlm.nih.gov
AH Nagi, W Kirkwood
Journal of clinical pathology, 1972ncbi.nlm.nih.gov
Method A fresh normal human kidney was obtained from a young man who died in a road
traffic accident. Its capsule was stripped off the cortex. The medulla was dissected away and
the cortical tissuewas cut into small pieces which were pushed through an 80-mesh metal
sieve using a spoon spatula. The kidney mesh was suspended in about 100 ml of cold
sterile normal saline. It was spun at 1000 rpm for three to four minutes and the supematant
was removed with a pasteur pipette. The wet glomerular pellet was then passed through a …
Method
A fresh normal human kidney was obtained from a young man who died in a road traffic accident. Its capsule was stripped off the cortex. The medulla was dissected away and the cortical tissuewas cut into small pieces which were pushed through an 80-mesh metal sieve using a spoon spatula. The kidney mesh was suspended in about 100 ml of cold sterile normal saline. It was spun at 1000 rpm for three to four minutes and the supematant was removed with a pasteur pipette. The wet glomerular pellet was then passed through a 150-mesh metal sieve and resuspended in about 50 to 75 ml sterile normal saline. The emerging suspension was rich in glomeruli most of which were without Bowman's capsule. The suspension was spun again at 1 000 rpm for three to four minutes and the supematant was removed with a pasteurpipette. The centrifugate was resuspended in normal saline and left standing in a refrigerator for about10 minutes. The super-natant was removed gently using a pasteur pipette and the deposit, which consisted of decapsulated
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