As a part of the examination of each intracranial tumor verified at operation in this clinic, tissue culture preparationsare made by the hanging drop methodI in order, if possible, to further our know-ledge of the histiogenesis of the tumors. Growths have been observed in the cultures of certain of the gliomas, acoustic nerve tumors, metastatic epithellal tumors, adenomas of the hypophysis and ependymal tumors. However, the best results and the most content in type of growth are obtained from the operative speimn of the glioblastoma multforme, t a rapidly growing invasive glioma of the cerebral hemispheres. This tumor is the most common type among the 496 verified and cassifiedgliomas studied in this cinic. Up to February I, 1929, there were r6o veri-fied examples of this type of glioma From ten to fifteen of these turs are available in this dinic for study h year. The purpose of this report is to present the results of the cultures made from the tissue of tumrs diagnosed as glioblastoma multi-forme, ten of which have been studied by the tissue culture method. Of these ten separate tumors, five showed excellent growth in vitro, butno oomparable growths were obtained from the remaining five although the same technique was used. Although many difficulties are met with in tissue culture preparations, it did seem that the lack of growth could be explained in some other manner. When the cinical histories and operative findings of the ten cases were studied, they were found to be similar to those of previously verified cases of the same type of gioma But when histological studies of the paraffin sections were made there was found a considerable vaia-tion in cell type and arrangement. The histological sections of the five cases showing excellent growth in culture showed quite similar