[HTML][HTML] TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages
Y Qiao, P Wang, J Qi, L Zhang, C Gao - FEBS letters, 2012 - Elsevier
Y Qiao, P Wang, J Qi, L Zhang, C Gao
FEBS letters, 2012•ElsevierNLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-
1β. However, the specific cis-and trans-regulatory elements that determine the extent of
NLRP3 expression are not well defined. In this study, we found NLRP3 expression was
induced by TLR agonists in murine macrophages in a NF-κB dependent manner.
Furthermore, the corresponding NF-κB binding sites (nt− 1303 to− 1292 and− 1238 to−
1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated …
1β. However, the specific cis-and trans-regulatory elements that determine the extent of
NLRP3 expression are not well defined. In this study, we found NLRP3 expression was
induced by TLR agonists in murine macrophages in a NF-κB dependent manner.
Furthermore, the corresponding NF-κB binding sites (nt− 1303 to− 1292 and− 1238 to−
1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated …
NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1β. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-κB dependent manner. Furthermore, the corresponding NF-κB binding sites (nt −1303 to −1292 and −1238 to −1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-κB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.
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