Microparticles are lipid bilayer–enclosed vesicles produced by cells under oxidative stress.
MP production is elevated in patients with diabetes, but the underlying cellular mechanisms
are poorly understood. We hypothesized that raising glucose above the physiological level
of 5.5 mm would stimulate leukocytes to produce MPs and activate the nucleotide-binding
domain, leucine-rich repeat pyrin domain–containing 3 (NLRP3) inflammasome. We found
that when incubated in buffer with up to 20 mm glucose, human and murine neutrophils, but …