RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells
NM Kretzer, DJ Theisen, R Tussiwand… - Journal of Experimental …, 2016 - rupress.org
Journal of Experimental Medicine, 2016•rupress.org
In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we
evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-
dependent CD8α+ and CD103+ compared with other DC subsets and immune lineages.
Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi
apparatus and LAMP1− cytoplasmic vesicles. Mice with germline or conditional deletion of
Rab43 are viable and fertile and have normal development of cDCs but show a defect for in …
evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-
dependent CD8α+ and CD103+ compared with other DC subsets and immune lineages.
Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi
apparatus and LAMP1− cytoplasmic vesicles. Mice with germline or conditional deletion of
Rab43 are viable and fertile and have normal development of cDCs but show a defect for in …
In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α+ and CD103+ compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1− cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a specialized activity used in cross-presentation selectively by CD8α+ DCs but not other antigen-presenting cells.
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