Identification of Aldophosphamide as a Metabolite of Cyclophosphamide in Vitro and in Vivo in Humans
C Fenselau, MNN Kan, SS Rao, A Myles, OM Friedman… - Cancer research, 1977 - AACR
C Fenselau, MNN Kan, SS Rao, A Myles, OM Friedman, M Colvin
Cancer research, 1977•AACRSummary Aldophosphamide (NSC 254), a putative key metabolite of cyclophosphamide,
has now been isolated as a cyanohydrin derivative from an incubation mixture of
cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a
cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an
authentic synthetic sample by mass spectrometry and combined gas chromatography-mass
spectrometry.
has now been isolated as a cyanohydrin derivative from an incubation mixture of
cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a
cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an
authentic synthetic sample by mass spectrometry and combined gas chromatography-mass
spectrometry.
Summary
Aldophosphamide (NSC 254), a putative key metabolite of cyclophosphamide, has now been isolated as a cyanohydrin derivative from an incubation mixture of cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an authentic synthetic sample by mass spectrometry and combined gas chromatography-mass spectrometry.
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