Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry
PP Chang, CG Vinuesa - T follicular Helper Cells: Methods and Protocols, 2015 - Springer
PP Chang, CG Vinuesa
T follicular Helper Cells: Methods and Protocols, 2015•SpringerNatural killer T follicular helper (NKT FH) cell, a recently identified B-cell helper innate cell
population, can be easily missed due to its low frequency and the fact that it only forms upon
immunization or infection with glycolipid-containing antigens or microbes. Here, we describe
our in-house optimized protocol to detect these mouse NKT FH cells by multiparameter flow
cytometer.
population, can be easily missed due to its low frequency and the fact that it only forms upon
immunization or infection with glycolipid-containing antigens or microbes. Here, we describe
our in-house optimized protocol to detect these mouse NKT FH cells by multiparameter flow
cytometer.
Abstract
Natural killer T follicular helper (NKTFH) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKTFH cells by multiparameter flow cytometer.
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