[HTML][HTML] Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa
OBJECTIVE: To examine simultaneously the levels of hydrogen peroxide (H2O2) and
superoxide (O2−•) using chemiluminescence and flow cytometry. DESIGN: Prospective
laboratory study. SETTING: Reproductive research lab in a tertiary hospital. PATIENT (S):
Semen samples from 18 healthy male volunteers. INTERVENTION (S): Sperm preparation
and measurement of reactive oxygen species (ROS) by chemiluminescence using luminol
and lucigenin before and after H2O2 exposure and by flow cytometry using …
superoxide (O2−•) using chemiluminescence and flow cytometry. DESIGN: Prospective
laboratory study. SETTING: Reproductive research lab in a tertiary hospital. PATIENT (S):
Semen samples from 18 healthy male volunteers. INTERVENTION (S): Sperm preparation
and measurement of reactive oxygen species (ROS) by chemiluminescence using luminol
and lucigenin before and after H2O2 exposure and by flow cytometry using …