Dendritic cell acquisition of epitope cargo mediated by simple cationic peptide structures

BY Chua, EM Eriksson, DP Poole, W Zeng… - Peptides, 2008 - Elsevier
In this study we evaluate the uptake by murine dendritic cells (DCs) of different synthetic,
branched cationic peptide structures with a view to facilitating peptide epitope delivery. The
level of cell uptake by fluorescenated peptides was measured by flow cytometry following
quenching of extracellular fluorescence with trypan blue. Branched peptides containing
either N-terminal arginine or N-terminal lysine residues were able to mediate cell entry but
the peptide containing four arginine residues in a branching configuration (R4) was found to …