[HTML][HTML] Studies on human DNA polymerase ϵ and GINS complex and their role in DNA replication

VP Bermudez, A Farina, V Raghavan, I Tappin… - Journal of Biological …, 2011 - ASBMB
In eukaryotic cells, DNA replication is carried out by the coordinated action of three DNA
polymerases (Pols), Pol α, δ, and ϵ. In this report, we describe the reconstitution of the
human four-subunit Pol ϵ and characterization of its catalytic properties in comparison with
Pol α and Pol δ. Human Pol ϵ holoenzyme is a monomeric complex containing
stoichiometric subunit levels of p261/Pol 2, p59, p17, and p12. We show that the Pol ϵ p261
N-terminal catalytic domain is solely responsible for its ability to catalyze DNA synthesis …