Characterization of lymphokine-activated killing by human peripheral blood mononuclear cells stimulated with interleukin 2 (IL-2) analogs specific for the intermediate …
KM Heaton, G Ju, DK Morris, K Delisio, P Bailon… - Cellular …, 1993 - Elsevier
Interleukin 2-stimulated human peripheral blood mononuclear cells (PBMC) generate
lymphokine-activated killing (LAK). Using the IL-2 analogs R38A and F42K, which interact
primarily with the β and γ subunits of the IL-2 receptor, we assessed the roles of IL-2Rβγ and
the high-affinity IL-2 receptor complex in LAK activation. Although the kinetics of LAK
activation were identical, lytic activity was approximately 30% lower and proliferation was up
to 55% lower in those PBMC stimulated by R38A or F42K than in those exposed to wild-type …
lymphokine-activated killing (LAK). Using the IL-2 analogs R38A and F42K, which interact
primarily with the β and γ subunits of the IL-2 receptor, we assessed the roles of IL-2Rβγ and
the high-affinity IL-2 receptor complex in LAK activation. Although the kinetics of LAK
activation were identical, lytic activity was approximately 30% lower and proliferation was up
to 55% lower in those PBMC stimulated by R38A or F42K than in those exposed to wild-type …