Macrophage migration inhibitory factor in lung tissue of idiopathic pulmonary fibrosis patients
C Olivieri, E Bargagli, S Inghilleri, I Campo… - Experimental Lung …, 2016 - Taylor & Francis
C Olivieri, E Bargagli, S Inghilleri, I Campo, M Cintorino, P Rottoli
Experimental Lung Research, 2016•Taylor & FrancisIntroduction: Idiopathic pulmonary fibrosis (IPF) is a severe interstitial lung disorder
characterized by a pattern of Usual Interstitial Pneumonia where the presence of fibroblastic
foci is the hallmark of the disease. Aim of the Study: In the present study, we analyzed the
migration inhibitory factor (MIF) expression in lung tissue of IPF patients compared with
healthy controls and organizing pneumonia (OP) patients focusing into MIF potential role in
fibroblastic foci development. Materials and Methods: The immunohistochemical analysis …
characterized by a pattern of Usual Interstitial Pneumonia where the presence of fibroblastic
foci is the hallmark of the disease. Aim of the Study: In the present study, we analyzed the
migration inhibitory factor (MIF) expression in lung tissue of IPF patients compared with
healthy controls and organizing pneumonia (OP) patients focusing into MIF potential role in
fibroblastic foci development. Materials and Methods: The immunohistochemical analysis …
Introduction
Idiopathic pulmonary fibrosis (IPF) is a severe interstitial lung disorder characterized by a pattern of Usual Interstitial Pneumonia where the presence of fibroblastic foci is the hallmark of the disease. Aim of the Study
In the present study, we analyzed the migration inhibitory factor (MIF) expression in lung tissue of IPF patients compared with healthy controls and organizing pneumonia (OP) patients focusing into MIF potential role in fibroblastic foci development. Materials and Methods
The immunohistochemical analysis was performed in 10 IPF patients (7 male), 3 OP patients (2 male), and 3 healthy controls (all male) using the streptavidin-biotin method (Dako). Results
In IPF samples, MIF resulted overexpressed in the areas of active fibrosis and, in particular, in the alveolar epithelium, bronchiolar epithelium, and in the peripheral zones of fibroblastic foci. Bronchiolar epithelium from organizing pneumonia patients resulted only weakly positive for MIF while no evidence of MIF expression was reported for alveolar epithelium. In the control subject group, MIF was unexpressed except for a weak presence in the bronchiolar epithelium. Conclusion
In conclusion, MIF is a pleiotropic cytokine involved in the pathogenesis of IPF being mainly expressed in the areas of remodeling and active fibrosis, in bronchiolar and alveolar epithelium, and in the peripheral zone of fibroblastic foci.Taylor & Francis Online