[HTML][HTML] Androgen receptor-mediated regulation of intracellular calcium in human airway smooth muscle cells
RSR Kalidhindi, R Katragadda… - Cellular physiology …, 2019 - ncbi.nlm.nih.gov
Cellular physiology and biochemistry: international journal of …, 2019•ncbi.nlm.nih.gov
Methods: We used primary human ASM cells from non-asthmatics vs. asthmatics to
determine AR expression at baseline and with inflammation measured using Western
blotting/qRT-PCR, and the role of AR in regulating intracellular Ca 2+([Ca 2+] i) measured
using Fluo-3 loaded real time [Ca 2+] i imaging. Results: We found that compared to
females, baseline AR is greater in male ASM and increases with inflammation/asthma.
Androgens, via AR, blunted TNFα or IL-13-induced enhancement of ASM [Ca 2+] i in both …
determine AR expression at baseline and with inflammation measured using Western
blotting/qRT-PCR, and the role of AR in regulating intracellular Ca 2+([Ca 2+] i) measured
using Fluo-3 loaded real time [Ca 2+] i imaging. Results: We found that compared to
females, baseline AR is greater in male ASM and increases with inflammation/asthma.
Androgens, via AR, blunted TNFα or IL-13-induced enhancement of ASM [Ca 2+] i in both …
Methods:
We used primary human ASM cells from non-asthmatics vs. asthmatics to determine AR expression at baseline and with inflammation measured using Western blotting/qRT-PCR, and the role of AR in regulating intracellular Ca 2+([Ca 2+] i) measured using Fluo-3 loaded real time [Ca 2+] i imaging.
Results:
We found that compared to females, baseline AR is greater in male ASM and increases with inflammation/asthma. Androgens, via AR, blunted TNFα or IL-13-induced enhancement of ASM [Ca 2+] i in both males and females, with retained efficacy in asthmatics. AR effects involve reduced Ca 2+ influx via L-type channels and store-operated Ca 2+ entry, the latter by downregulating STIM1 and Orai1 and increasing TMEM66.
Conclusion:
Our data show AR expression is increased in female ASM with asthma, but has retained functionality that could be used to reduce [Ca 2+] i towards alleviating airway hyperresponsiveness.
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