Tumor necrosis factor (TNF)-α induction of CXCL10 in endothelial cells requires protein arginine methyltransferase 5 (PRMT5)-mediated nuclear factor (NF)-κB p65 …
DP Harris, S Bandyopadhyay, TJ Maxwell… - Journal of Biological …, 2014 - jbc.org
The chemokine CXCL10/IP-10 facilitates recruitment of Th1-type leukocytes to inflammatory
sites. In this study, we show that the arginine methyltransferase PRMT5 is critical for
CXCL10 transcription in TNF-α-activated human endothelial cells (EC). We found that
depletion of PRMT5 results in significantly reduced levels of CXCL10 mRNA, demonstrating
a positive role for PRMT5 in CXCL10 induction. Chromatin immunoprecipitation experiments
revealed the presence of the symmetrical dimethylarginine modification catalyzed by …
sites. In this study, we show that the arginine methyltransferase PRMT5 is critical for
CXCL10 transcription in TNF-α-activated human endothelial cells (EC). We found that
depletion of PRMT5 results in significantly reduced levels of CXCL10 mRNA, demonstrating
a positive role for PRMT5 in CXCL10 induction. Chromatin immunoprecipitation experiments
revealed the presence of the symmetrical dimethylarginine modification catalyzed by …
