Isolation of Clara cells from the mouse lung.
VI Oreffo, A Morgan, RJ Richards - Environmental health …, 1990 - ehp.niehs.nih.gov
A method is described for isolating Clara cells from the mouse lung that does not require the
technique of elutriation. Mouse lungs totally perfused of blood are instilled with crystalline
trypsin (0.25%) and incubated for the optimum time of 15 min. The lung tissue is chopped,
mechanically agitated, and sequentially filtered to obtain a primary digest of 3 to 5 x 10 (6)
cells. Clara cells, identified routinely by histochemical localization of NADPH diaphorase,
using the stain nitrotetrazolium blue (NBT), accounts for between 20 to 40% of the cells in …
technique of elutriation. Mouse lungs totally perfused of blood are instilled with crystalline
trypsin (0.25%) and incubated for the optimum time of 15 min. The lung tissue is chopped,
mechanically agitated, and sequentially filtered to obtain a primary digest of 3 to 5 x 10 (6)
cells. Clara cells, identified routinely by histochemical localization of NADPH diaphorase,
using the stain nitrotetrazolium blue (NBT), accounts for between 20 to 40% of the cells in …