[PDF][PDF] Endocytosis and recycling of immune complexes by follicular dendritic cells enhances B cell antigen binding and activation

BA Heesters, P Chatterjee, YA Kim, SF Gonzalez… - Immunity, 2013 - cell.com
BA Heesters, P Chatterjee, YA Kim, SF Gonzalez, MP Kuligowski, T Kirchhausen, MC Carroll
Immunity, 2013cell.com
Stromal-derived follicular dendritic cells (FDCs) are a major reservoir for antigen that are
essential for formation of germinal centers, the site where memory and effector B cells
differentiate. A long-standing question is how FDCs retain antigen in its native form for
extended periods and how they display it to specific B cells. Here we found that FDCs
acquired complement-coated immune complexes (ICs) from noncognate B cells via
complement receptors 1 and 2 (CD35 and CD21, respectively) and rapidly internalized them …
Summary
Stromal-derived follicular dendritic cells (FDCs) are a major reservoir for antigen that are essential for formation of germinal centers, the site where memory and effector B cells differentiate. A long-standing question is how FDCs retain antigen in its native form for extended periods and how they display it to specific B cells. Here we found that FDCs acquired complement-coated immune complexes (ICs) from noncognate B cells via complement receptors 1 and 2 (CD35 and CD21, respectively) and rapidly internalized them by an actin-dependent pathway. ICs were retained intact within a nondegradative cycling compartment and were displayed periodically on the cell surface where they were accessible to antigen-specific B cells. This would explain how antigens are protected from damage and retained over long periods of time, while remaining accessible for B cells.
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