[HTML][HTML] Acute loss of iron–sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells
DR Crooks, N Maio, AN Lane, M Jarnik… - Journal of Biological …, 2018 - ASBMB
Iron–sulfur (Fe-S) clusters are ancient cofactors in cells and participate in diverse
biochemical functions, including electron transfer and enzymatic catalysis. Although cell
lines derived from individuals carrying mutations in the Fe-S cluster biogenesis pathway or
siRNA-mediated knockdown of the Fe-S assembly components provide excellent models for
investigating Fe-S cluster formation in mammalian cells, these experimental strategies focus
on the consequences of prolonged impairment of Fe-S assembly. Here, we constructed and …
biochemical functions, including electron transfer and enzymatic catalysis. Although cell
lines derived from individuals carrying mutations in the Fe-S cluster biogenesis pathway or
siRNA-mediated knockdown of the Fe-S assembly components provide excellent models for
investigating Fe-S cluster formation in mammalian cells, these experimental strategies focus
on the consequences of prolonged impairment of Fe-S assembly. Here, we constructed and …