C/ATF, a member of the activating transcription factor family of DNA-binding proteins, dimerizes with CAAT/enhancer-binding proteins and directs their binding to …

M Vallejo, D Ron, CP Miller… - Proceedings of the …, 1993 - National Acad Sciences
M Vallejo, D Ron, CP Miller, JF Habener
Proceedings of the National Academy of Sciences, 1993National Acad Sciences
Members of the C/EBP family of basic-leucine zipper (bZip) transcription factors form
heterodimers and bind to the CAAT box and other sequence-related enhancer motifs. Using
a 32P-labeled protein probe consisting of the bZip domain of C/EBP beta, we isolated a
clone encoding C/EBP-related ATF (C/ATF), a bZip protein that heterodimerizes with C/EBP-
like proteins but belongs to the CREB/ATF family. C/ATF homodimers do not bind to typical
C/EBP DNA sites. Instead they bind to palindromic cAMP response elements such as that of …
Members of the C/EBP family of basic-leucine zipper (bZip) transcription factors form heterodimers and bind to the CAAT box and other sequence-related enhancer motifs. Using a 32P-labeled protein probe consisting of the bZip domain of C/EBP beta, we isolated a clone encoding C/EBP-related ATF (C/ATF), a bZip protein that heterodimerizes with C/EBP-like proteins but belongs to the CREB/ATF family. C/ATF homodimers do not bind to typical C/EBP DNA sites. Instead they bind to palindromic cAMP response elements such as that of the somatostatin gene. In addition, C/ATF-C/EBP beta heterodimers bind to a subclass of asymmetric cAMP response elements exemplified by those in the phosphoenolpyruvate carboxykinase and proenkephalin genes. Transient transfection studies indicate that interactions between C/ATF and C/EBP beta are the basis for a functional cross talk between these two families of transcription factors that may be important for the integration of hormonal and developmental stimuli that determine the expression of subsets of genes in specific cellular phenotypes.
National Acad Sciences