Detection and tracking of dual-labeled HIV particles using wide-field live cell imaging to follow viral core integrity
Live cell imaging is a valuable technique that allows the characterization of the dynamic
processes of the HIV-1 life cycle. Here, we present a method of production and imaging of
dual-labeled HIV viral particles that allows the visualization of two events. Varying release of
the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral
cores with the use of live wide-field fluorescent microscopy.
processes of the HIV-1 life cycle. Here, we present a method of production and imaging of
dual-labeled HIV viral particles that allows the visualization of two events. Varying release of
the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral
cores with the use of live wide-field fluorescent microscopy.
Abstract
Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.
Springer