Interleukin-6 and cAMP induce stromal cell-derived factor-1 chemotaxis in astroglia by up-regulating CXCR4 cell surface expression: implications for brain …
The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 control the
migration of neurons and microglial cells in the central nervous system. Although functional
CXCR4 is also expressed by astroglia, recent studies have failed to observe a chemotactic
response of these cells to SDF-1. Here, we demonstrate that SDF-1-dependent chemotaxis
can be induced by treating cultured cortical astroglia with either dibutyryl cyclic AMP
(dbcAMP; 10− 4 m) or interleukin-6 (IL-6; 10 ng/ml). Flow cytometric analysis revealed that …
migration of neurons and microglial cells in the central nervous system. Although functional
CXCR4 is also expressed by astroglia, recent studies have failed to observe a chemotactic
response of these cells to SDF-1. Here, we demonstrate that SDF-1-dependent chemotaxis
can be induced by treating cultured cortical astroglia with either dibutyryl cyclic AMP
(dbcAMP; 10− 4 m) or interleukin-6 (IL-6; 10 ng/ml). Flow cytometric analysis revealed that …