Transposable elements have rewired the core regulatory network of human embryonic stem cells
Nature genetics, 2010•nature.com
Detection of new genomic control elements is critical in understanding transcriptional
regulatory networks in their entirety. We studied the genome-wide binding locations of three
key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and
mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4
and NANOG are markedly different, with only∼ 5% of the regions being homologously
occupied. We show that transposable elements contributed up to 25% of the bound sites in …
regulatory networks in their entirety. We studied the genome-wide binding locations of three
key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and
mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4
and NANOG are markedly different, with only∼ 5% of the regions being homologously
occupied. We show that transposable elements contributed up to 25% of the bound sites in …
Abstract
Detection of new genomic control elements is critical in understanding transcriptional regulatory networks in their entirety. We studied the genome-wide binding locations of three key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4 and NANOG are markedly different, with only ∼5% of the regions being homologously occupied. We show that transposable elements contributed up to 25% of the bound sites in humans and mice and have wired new genes into the core regulatory network of embryonic stem cells. These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells.
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