Estrogen regulation of tissue-specific expression of complement C3

SA Sundstrom, BS Komm, H Ponce-de-Leon… - Journal of Biological …, 1989 - ASBMB
The administration of estradiol to immature rats results in the increased synthesis and
secretion of a 180-kDa protein, composed of 115-and 65-kDa subunits, by the uterine
luminal epithelial cells. A monoclonal antibody against the 180-kDa protein was utilized to
isolate the corresponding cDNA (LE-1) from a rat uterine luminal epithelial cell cDNA λgt11
expression library. This LE-1 cDNA was sequenced and shown to be homologous to
complement component C3. The sequence was approximately 81 and 90% homologous to …