Verapamil inhibits ureteral scar formation by regulating CaMK II-mediated Smad pathway
MQ Zeng, W Xiao, K Yang, ZY Gao, JS Wang… - Chemico-Biological …, 2021 - Elsevier
Verapamil is reported to prevent scar formation. However, whether verapamil is involved in
the ureteral stricture scar and the underlying mechanism need further investigation.
Fibroblasts were isolated from ureteral scar tissues. TGF-β1 stimulation was used to induce
fibrosis of fibroblasts. Inhibition of CaMK II was achieved by shRNA transfection. CCK-8 was
performed to evaluate cell viability. qRT-PCR was applied to determine the level of mRNA
while western blotting was used to determine the level of proteins. Immunofluorescence was …
the ureteral stricture scar and the underlying mechanism need further investigation.
Fibroblasts were isolated from ureteral scar tissues. TGF-β1 stimulation was used to induce
fibrosis of fibroblasts. Inhibition of CaMK II was achieved by shRNA transfection. CCK-8 was
performed to evaluate cell viability. qRT-PCR was applied to determine the level of mRNA
while western blotting was used to determine the level of proteins. Immunofluorescence was …
