A dynamic spectrum of monocytes arising from the in situ reprogramming of CCR2+ monocytes at a site of sterile injury

D Dal-Secco, J Wang, Z Zeng… - Journal of Experimental …, 2015 - rupress.org
D Dal-Secco, J Wang, Z Zeng, E Kolaczkowska, CHY Wong, B Petri, RM Ransohoff
Journal of Experimental Medicine, 2015rupress.org
Monocytes are recruited from the blood to sites of inflammation, where they contribute to
wound healing and tissue repair. There are at least two subsets of monocytes: classical or
proinflammatory (CCR2hiCX3CR1low) and nonclassical, patrolling, or alternative
(CCR2lowCX3CR1hi) monocytes. Using spinning-disk confocal intravital microscopy and
mice with fluorescent reporters for each of these subsets, we were able to track the dynamic
spectrum of monocytes that enter a site of sterile hepatic injury in vivo. We observed that the …
Monocytes are recruited from the blood to sites of inflammation, where they contribute to wound healing and tissue repair. There are at least two subsets of monocytes: classical or proinflammatory (CCR2hiCX3CR1low) and nonclassical, patrolling, or alternative (CCR2lowCX3CR1hi) monocytes. Using spinning-disk confocal intravital microscopy and mice with fluorescent reporters for each of these subsets, we were able to track the dynamic spectrum of monocytes that enter a site of sterile hepatic injury in vivo. We observed that the CCR2hiCX3CR1low monocytes were recruited early and persisted for at least 48 h, forming a ringlike structure around the injured area. These monocytes transitioned, in situ, from CCR2hiCx3CR1low to CX3CR1hiCCR2low within the ringlike structure and then entered the injury site. This phenotypic conversion was essential for optimal repair. These results demonstrate a local, cytokine driven reprogramming of classic, proinflammatory monocytes into nonclassical or alternative monocytes to facilitate proper wound-healing.
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