DNase1 does not appear to play a major role in the fragmentation of plasma DNA in a knockout mouse model
Clinical chemistry, 2018•academic.oup.com
There has been much recent interest in the biology of plasma DNA because of the
emergence of noninvasive prenatal testing and liquid biopsy for cancer. Circulating cell-free
(cf) DNA displays distinct fragmentation patterns in terms of fragment sizes, genomic
distribution, and fragment endpoints (1, 2). Although the mechanism of cfDNA fragmentation
remains largely unknown, fragment characteristics can be used to infer epigenetic
architecture (3) and gene expression (4) at the site of DNA origin. DNase1 is a major …
emergence of noninvasive prenatal testing and liquid biopsy for cancer. Circulating cell-free
(cf) DNA displays distinct fragmentation patterns in terms of fragment sizes, genomic
distribution, and fragment endpoints (1, 2). Although the mechanism of cfDNA fragmentation
remains largely unknown, fragment characteristics can be used to infer epigenetic
architecture (3) and gene expression (4) at the site of DNA origin. DNase1 is a major …
There has been much recent interest in the biology of plasma DNA because of the emergence of noninvasive prenatal testing and liquid biopsy for cancer. Circulating cell-free (cf) DNA displays distinct fragmentation patterns in terms of fragment sizes, genomic distribution, and fragment endpoints (1, 2). Although the mechanism of cfDNA fragmentation remains largely unknown, fragment characteristics can be used to infer epigenetic architecture (3) and gene expression (4) at the site of DNA origin. DNase1 is a major secretory nuclease. We used a DNase1 knockout
Oxford University Press