Proplatelet formation dynamics of mouse fresh bone marrow explants
I Guinard, F Lanza, C Gachet, C Léon… - Journal of Visualized …, 2021 - app.jove.com
The last stage of megakaryopoiesis leads to cytoplasmic extensions from mature
megakaryocytes, the so-called proplatelets. Much has been learned about the proplatelet
formation using in vitro-differentiated megakaryocytes; however, there is an increasing
evidence that conventional culture systems do not faithfully recapitulate the differentiation/
maturation process that takes places inside the bone marrow. In this manuscript, we present
an explant method initially described in 1956 by Thiéry and Bessis to visualize …
megakaryocytes, the so-called proplatelets. Much has been learned about the proplatelet
formation using in vitro-differentiated megakaryocytes; however, there is an increasing
evidence that conventional culture systems do not faithfully recapitulate the differentiation/
maturation process that takes places inside the bone marrow. In this manuscript, we present
an explant method initially described in 1956 by Thiéry and Bessis to visualize …