Identification of protein-protein interactions is crucial for unraveling cellular processes and
biochemical mechanisms of signal transduction. Here we describe, for the first time, the
application of the tandem affinity purification (TAP) and LC-MS method to the
characterization of protein complexes from transgenic mice. The TAP strategy developed in
transgenic mice allows the emplacement of complexes in their physiological environment in
contact with proteins that might only be specifically expressed in certain tissues while …