[HTML][HTML] Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets
Diabetologia, 2015•Springer
Aims/hypothesis Insulin secretion is widely studied because it plays a central role in glucose
homeostasis and diabetes. Processes from insulin granule fusion in beta cells to in vivo
insulin secretion have been elucidated, but data at the cellular level do not fully account for
several aspects of the macroscopic secretory pattern. Here we investigated how individual
secretory events are coordinated spatially and temporally within intact human islets.
Methods We used the fluorescent probe neuropeptide Y (NPY)–pHluorin to visualise insulin …
homeostasis and diabetes. Processes from insulin granule fusion in beta cells to in vivo
insulin secretion have been elucidated, but data at the cellular level do not fully account for
several aspects of the macroscopic secretory pattern. Here we investigated how individual
secretory events are coordinated spatially and temporally within intact human islets.
Methods We used the fluorescent probe neuropeptide Y (NPY)–pHluorin to visualise insulin …
Aims/hypothesis
Insulin secretion is widely studied because it plays a central role in glucose homeostasis and diabetes. Processes from insulin granule fusion in beta cells to in vivo insulin secretion have been elucidated, but data at the cellular level do not fully account for several aspects of the macroscopic secretory pattern. Here we investigated how individual secretory events are coordinated spatially and temporally within intact human islets.
Methods
We used the fluorescent probe neuropeptide Y (NPY)–pHluorin to visualise insulin granule secretion in isolated intact human islets.
Results
We found that individual beta cells respond to increases in glucose concentration by releasing insulin granules in very discrete bursts with periods consistent with in vivo pulsatile insulin secretion. In successive secretory bursts during prolonged exposure to high glucose levels, secretory events progressively localised to preferential release sites, coinciding with the transition to second phase insulin secretion. Granule secretion was very synchronised in neighbouring beta cells, forming discrete regional clusters of activity.
Conclusions/interpretation
These results reveal how individual secretory events are coordinated to produce pulsatile insulin secretion from human islets.
Springer