Interest in evaluating individual cellular populations in the central nervous system has prompted the development of several techniques enabling the enrichment of single‐cell populations. Herein we detail a relatively inexpensive method to specifically isolate neurons, astrocytes, and microglia from a mixed homogenate utilizing magnetic beads conjugated to cell‐type specific antibodies. We have used this technique to isolate astrocytes across development and into late adulthood. Finally, we detail the utilization of this technique in novel astrocyte and astrocyte/neuron co‐culture paradigms. © 2019 by John Wiley & Sons, Inc.