An open-access microfluidic model for lung-specific functional studies at an air-liquid interface
DD Nalayanda, C Puleo, WB Fulton, LM Sharpe… - Biomedical …, 2009 - Springer
Biomedical microdevices, 2009•Springer
In an effort to improve the physiologic relevance of existing in vitro models for alveolar cells,
we present a microfluidic platform which provides an air-interface in a dynamic system
combining microfluidic and suspended membrane culture systems. Such a system provides
the ability to manipulate multiple parameters on a single platform along with ease in cell
seeding and manipulation. The current study presents a comparison of the efficacy of the
hybrid system with conventional platforms using assays analyzing the maintenance of …
we present a microfluidic platform which provides an air-interface in a dynamic system
combining microfluidic and suspended membrane culture systems. Such a system provides
the ability to manipulate multiple parameters on a single platform along with ease in cell
seeding and manipulation. The current study presents a comparison of the efficacy of the
hybrid system with conventional platforms using assays analyzing the maintenance of …
Abstract
In an effort to improve the physiologic relevance of existing in vitro models for alveolar cells, we present a microfluidic platform which provides an air-interface in a dynamic system combining microfluidic and suspended membrane culture systems. Such a system provides the ability to manipulate multiple parameters on a single platform along with ease in cell seeding and manipulation. The current study presents a comparison of the efficacy of the hybrid system with conventional platforms using assays analyzing the maintenance of function and integrity of A549 alveolar epithelial cell monolayer cultures. The hybrid system incorporates bio-mimetic nourishment on the basal side of the epithelial cells along with an open system on the apical side of the cells exposed to air allowing for easy access for assays.
Springer