Primary culture of parenchymal liver cells on collagen membranes: morphological and biochemical observations
G Michalopoulos, HC Pitot - Experimental cell research, 1975 - Elsevier
G Michalopoulos, HC Pitot
Experimental cell research, 1975•ElsevierComparative morphological and biochemical studies of primary cultures of parenchymal
liver cells from adult rat liver cultured on collagen-coated plates and floating collagen
membranes indicate that the latter have a markedly prolonged viability and show
morphological and functional features reminiscent of liver in vivo. Tyrosine aminotransferase
induction by cyclic nucleotides or steroids can be maintained in liver cells cultured on
floating collagen membranes for periods of 3 weeks. The advantages of this system over …
liver cells from adult rat liver cultured on collagen-coated plates and floating collagen
membranes indicate that the latter have a markedly prolonged viability and show
morphological and functional features reminiscent of liver in vivo. Tyrosine aminotransferase
induction by cyclic nucleotides or steroids can be maintained in liver cells cultured on
floating collagen membranes for periods of 3 weeks. The advantages of this system over …
Abstract
Comparative morphological and biochemical studies of primary cultures of parenchymal liver cells from adult rat liver cultured on collagen-coated plates and floating collagen membranes indicate that the latter have a markedly prolonged viability and show morphological and functional features reminiscent of liver in vivo. Tyrosine aminotransferase induction by cyclic nucleotides or steroids can be maintained in liver cells cultured on floating collagen membranes for periods of 3 weeks. The advantages of this system over those previously reported for the cultivation of liver cells is discussed.
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