Exercise-induced changes in metabolic intermediates, hormones, and inflammatory markers associated with improvements in insulin sensitivity
KM Huffman, CA Slentz, LA Bateman… - Diabetes …, 2011 - Am Diabetes Assoc
Diabetes Care, 2011•Am Diabetes Assoc
OBJECTIVE To understand relationships between exercise training-mediated improvements
in insulin sensitivity (SI) and changes in circulating concentrations of metabolic
intermediates, hormones, and inflammatory mediators. RESEARCH DESIGN AND
METHODS Targeted mass spectrometry and enzyme-linked immunosorbent assays were
used to quantify metabolic intermediates, hormones, and inflammatory markers at baseline,
after 6 months of exercise training, and 2 weeks after exercise training cessation (n= 53). A …
in insulin sensitivity (SI) and changes in circulating concentrations of metabolic
intermediates, hormones, and inflammatory mediators. RESEARCH DESIGN AND
METHODS Targeted mass spectrometry and enzyme-linked immunosorbent assays were
used to quantify metabolic intermediates, hormones, and inflammatory markers at baseline,
after 6 months of exercise training, and 2 weeks after exercise training cessation (n= 53). A …
OBJECTIVE
To understand relationships between exercise training-mediated improvements in insulin sensitivity (SI) and changes in circulating concentrations of metabolic intermediates, hormones, and inflammatory mediators.
RESEARCH DESIGN AND METHODS
Targeted mass spectrometry and enzyme-linked immunosorbent assays were used to quantify metabolic intermediates, hormones, and inflammatory markers at baseline, after 6 months of exercise training, and 2 weeks after exercise training cessation (n = 53). A principal components analysis (PCA) strategy was used to relate changes in these intermediates to changes in SI.
RESULTS
PCA reduced the number of intermediates from 90 to 24 factors composed of biologically related components. With exercise training, improvements in SI were associated with reductions in by-products of fatty acid oxidation and increases in glycine and proline (P < 0.05, R2 = 0.59); these relationships were retained 15 days after cessation of exercise training (P < 0.05, R2 = 0.34).
CONCLUSIONS
These observations support prior observations in animal models that exercise training promotes more efficient mitochondrial β-oxidation and challenges current hypotheses regarding exercise training and glycine metabolism.
Am Diabetes Assoc