[HTML][HTML] Role of innate lymphoid cells in chronic colitis during anti-IL-17A therapy

CH Park, A Lee, SB Ahn, CS Eun, DS Han - Scientific Reports, 2020 - nature.com
CH Park, A Lee, SB Ahn, CS Eun, DS Han
Scientific Reports, 2020nature.com
IL-17A is an important cytokine in intestinal inflammation. However, anti-IL-17A therapy does
not improve clinical outcomes in patients with Crohn's disease. We aimed to evaluate the
role of RORγt+ innate lymphoid cells (ILCs) in murine colitis models in the absence of IL-
17A. An acute colitis model was induced with either dextran sulfate sodium (DSS) or
trinitrobenzenesulfonic acid (TNBS) and a chronic colitis model was induced by CD4+
CD45RBhi T cell transfer from either wild-type C57BL/6 or Il17a−/− mice. An anti-IL-17A …
Abstract
IL-17A is an important cytokine in intestinal inflammation. However, anti-IL-17A therapy does not improve clinical outcomes in patients with Crohn’s disease. We aimed to evaluate the role of RORγt+ innate lymphoid cells (ILCs) in murine colitis models in the absence of IL-17A. An acute colitis model was induced with either dextran sulfate sodium (DSS) or trinitrobenzenesulfonic acid (TNBS) and a chronic colitis model was induced by CD4+CD45RBhi T cell transfer from either wild-type C57BL/6 or Il17a−/− mice. An anti-IL-17A antibody, secukinumab, was also used to inhibit IL-17A function in the colitis model. Flow cytometry was performed to analyze the population of RORγt+ ILCs in the colonic lamina propria of mice with chronic colitis. Acute intestinal inflammation due to DSS and TNBS was attenuated in IL-17A knockout mice, whereas chronic colitis was not relieved by T cell transfer from Il17a−/− mice (% of original body weight: wild-type mice vs. Il17a−/− mice, 81.9% vs. 82.2%; P = 0.922). However, the mean proportion of Lin-RORγt+ lymphocytes was higher after T cell transfer from Il17a−/− mice than that after T cell transfer from wild-type mice (28.8% vs. 18.5%). The proportion of Lin-RORγt+ was also increased in Rag2−/− mice that received T cell transfer from wild-type mice when anti-IL-17A antibody was administered (31.7%). Additionally, Il6 and Il22 tended to be highly expressed after T cell transfer from Il17a−/− mice. In conclusion, RORγt+ ILCs may have an important role in the pathogenesis of chronic colitis in the absence of IL-17A. Blocking the function of IL-17A may upregulate Il6 and recruit RORγt+ ILCs in chronic colitis, thereby upregulating IL-22 and worsening the clinical outcomes of patients with Crohn’s disease.
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