Phosphoinositide-dependent kinase 1 and mTORC2 synergistically maintain postnatal heart growth and heart function in mice

X Zhao, S Lu, J Nie, X Hu, W Luo, X Wu… - … and Cellular Biology, 2014 - Am Soc Microbiol
X Zhao, S Lu, J Nie, X Hu, W Luo, X Wu, H Liu, Q Feng, Z Chang, Y Liu, Y Cao, H Sun, X Li…
Molecular and Cellular Biology, 2014Am Soc Microbiol
The protein kinase Akt plays a critical role in heart function and is activated by
phosphorylation of threonine 308 (T308) and serine 473 (S473). While phosphoinositide-
dependent kinase 1 (PDK1) is responsible for Akt T308 phosphorylation, the identities of the
kinases for Akt S473 phosphorylation in the heart remain controversial. Here, we disrupted
mTOR complex 2 (mTORC2) through deletion of Rictor in the heart and found normal heart
growth and function. Rictor deletion caused significant reduction of Akt S473 …
Abstract
The protein kinase Akt plays a critical role in heart function and is activated by phosphorylation of threonine 308 (T308) and serine 473 (S473). While phosphoinositide-dependent kinase 1 (PDK1) is responsible for Akt T308 phosphorylation, the identities of the kinases for Akt S473 phosphorylation in the heart remain controversial. Here, we disrupted mTOR complex 2 (mTORC2) through deletion of Rictor in the heart and found normal heart growth and function. Rictor deletion caused significant reduction of Akt S473 phosphorylation but enhanced Akt T308 phosphorylation, suggesting that a high level of Akt T308 phosphorylation maintains Akt activity and heart function. Deletion of Pdk1 in the heart caused significantly enhanced Akt S473 phosphorylation that was suppressed by removal of Rictor, leading to worsened dilated cardiomyopathy (DCM) and accelerated heart failure in Pdk1-deficient mice. In addition, we found that increasing Akt S473 phosphorylation through deletion of Pten or chemical inhibition of PTEN reversed DCM and heart failure in Pdk1-deficient mice. Investigation of heart samples from human DCM patients revealed changes similar to those in the mouse models. These results demonstrated that PDK1 and mTORC2 synergistically promote postnatal heart growth and maintain heart function in postnatal mice.
American Society for Microbiology