Background In the last few decades, zebrafish (Danio rerio) were introduced as a model
organism to investigate human diseases including cardiovascular and neuronal disorders.
In most zebrafish investigations, cardiac function and blood flow hemodynamics need to be
assessed to study the effects of the interference on the cardiovascular system. For heart
function assessment, most important parameters include heart rate, cardiac output, ejection
fraction, fractional area change, and fractional shortening. Methods A 10 s high-speed video …