[HTML][HTML] The endoplasmic reticulum Grp170 acts as a nucleotide exchange factor of Hsp70 via a mechanism similar to that of the cytosolic Hsp110
C Andréasson, H Rampelt, J Fiaux… - Journal of biological …, 2010 - ASBMB
Grp170 and Hsp110 proteins constitute two evolutionary distinct branches of the Hsp70
family that share the ability to function as nucleotide exchange factors (NEFs) for canonical
Hsp70s. Although the NEF mechanism of the cytoplasmic Hsp110s is well understood, little
is known regarding the mechanism used by Grp170s in the endoplasmic reticulum. In this
study, we compare the yeast Grp170 Lhs1 with the yeast Hsp110 Sse1. We find that
residues important for Sse1 NEF activity are conserved in Lhs1 and that mutations in these …
family that share the ability to function as nucleotide exchange factors (NEFs) for canonical
Hsp70s. Although the NEF mechanism of the cytoplasmic Hsp110s is well understood, little
is known regarding the mechanism used by Grp170s in the endoplasmic reticulum. In this
study, we compare the yeast Grp170 Lhs1 with the yeast Hsp110 Sse1. We find that
residues important for Sse1 NEF activity are conserved in Lhs1 and that mutations in these …