Preparation of genomic DNA from mammalian tissue
WM Strauss - Current protocols in molecular biology, 1998 - Wiley Online Library
WM Strauss
Current protocols in molecular biology, 1998•Wiley Online LibraryThere are a number of different procedures for the preparation of genomic DNA. They all
start with some form of cell lysis, followed by deproteination and recovery of DNA. The main
differences between various approaches lie in the extent of deproteination and in molecular
weight of the DNA produced. The isolation procedure described here is relatively brief and
relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability
of the ionic detergent SDS.
start with some form of cell lysis, followed by deproteination and recovery of DNA. The main
differences between various approaches lie in the extent of deproteination and in molecular
weight of the DNA produced. The isolation procedure described here is relatively brief and
relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability
of the ionic detergent SDS.
Abstract
There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteination and recovery of DNA. The main differences between various approaches lie in the extent of deproteination and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.
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