[HTML][HTML] Baicalin attenuates oxygen-glucose deprivation-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase activation in PC12 cells
C Li, W Zhang, S Fang, Y Lu, L Zhang, L Qi… - Acta pharmacologica …, 2010 - nature.com
C Li, W Zhang, S Fang, Y Lu, L Zhang, L Qi, X Huang, X Huang, E Wei
Acta pharmacologica sinica, 2010•nature.comAim: To determine whether the flavonoid baicalin attenuates oxygen-glucose deprivation
(OGD)-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase (5-LOX)
activation in PC12 cells. Methods: The effects of baicalin and the 5-LOX inhibitor zileuton on
the changes induced by OGD/recovery or H 2 O 2 (an exogenous reactive oxygen species
[ROS]) in green fluorescent protein-5-LOX-transfected PC12 cells were compared. Results:
Both baicalin and zileuton attenuated OGD/recovery-and H 2 O 2-induced injury and …
(OGD)-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase (5-LOX)
activation in PC12 cells. Methods: The effects of baicalin and the 5-LOX inhibitor zileuton on
the changes induced by OGD/recovery or H 2 O 2 (an exogenous reactive oxygen species
[ROS]) in green fluorescent protein-5-LOX-transfected PC12 cells were compared. Results:
Both baicalin and zileuton attenuated OGD/recovery-and H 2 O 2-induced injury and …
Abstract
Aim:
To determine whether the flavonoid baicalin attenuates oxygen-glucose deprivation (OGD)-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase (5-LOX) activation in PC12 cells.
Methods:
The effects of baicalin and the 5-LOX inhibitor zileuton on the changes induced by OGD/recovery or H 2 O 2 (an exogenous reactive oxygen species [ROS]) in green fluorescent protein-5-LOX-transfected PC12 cells were compared.
Results:
Both baicalin and zileuton attenuated OGD/recovery-and H 2 O 2-induced injury and inhibited OGD/recovery-induced production of 5-LOX metabolites (cysteinyl leukotrienes) in a concentration-dependent manner. However, baicalin did not reduce baseline cysteinyl leukotriene levels. Baicalin also reduced OGD/recovery-induced ROS production and inhibited 5-LOX translocation to the nuclear envelope and p38 phosphorylation induced by OGD/recovery and H 2 O 2. In contrast, zileuton did not show these effects.
Conclusion:
Baicalin can inhibit 5-LOX activation after ischemic injury, which may partly result from inhibition of the ROS/p38 mitogen-activated protein kinase pathway.
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