An intracellular interaction network regulates conformational transitions in the dopamine transporter
Neurotransmitter: sodium symporters (NSS) 1 mediate sodium-dependent reuptake of
neurotransmitters from the synaptic cleft and are targets for many psychoactive drugs. The
crystal structure of the prokaryotic NSS protein, LeuT, was recently solved at high resolution;
however, the mechanistic details of regulation of the permeation pathway in this class of
proteins remain unknown. Here we combine computational modeling and experimental
probing in the dopamine transporter (DAT) to demonstrate the functional importance of a …
neurotransmitters from the synaptic cleft and are targets for many psychoactive drugs. The
crystal structure of the prokaryotic NSS protein, LeuT, was recently solved at high resolution;
however, the mechanistic details of regulation of the permeation pathway in this class of
proteins remain unknown. Here we combine computational modeling and experimental
probing in the dopamine transporter (DAT) to demonstrate the functional importance of a …
