Optimization of methods to assess human mucosal T-cell responses to HIV infection
BL Shacklett, O Yang, MA Hausner, J Elliott… - Journal of …, 2003 - Elsevier
The majority of HIV-1 infections occur via sexual transmission at mucosal epithelia lining the
vagina, cervix or rectum. Mucosal tissues also serve as viral reservoirs. However, our
knowledge of human mucosal T-cell responses is limited. There is a need for reliable,
sensitive, and reproducible methods for assessing mucosal immunity. Here we report on the
collaborative efforts of two laboratories to optimize methods for processing, culturing, and
analyzing mucosal lymphocytes. Rectal biopsy tissue was obtained by flexible …
vagina, cervix or rectum. Mucosal tissues also serve as viral reservoirs. However, our
knowledge of human mucosal T-cell responses is limited. There is a need for reliable,
sensitive, and reproducible methods for assessing mucosal immunity. Here we report on the
collaborative efforts of two laboratories to optimize methods for processing, culturing, and
analyzing mucosal lymphocytes. Rectal biopsy tissue was obtained by flexible …