Analysis of gene expression and gene silencing in human macrophages
FO Martinez - Current protocols in immunology, 2012 - Wiley Online Library
Current protocols in immunology, 2012•Wiley Online Library
This unit describes how to execute a gene expression study with human macrophages. It
includes protocols for human macrophage preparation, RNA extraction, real‐time PCR
analysis, and microarray analysis. The unit also includes a protocol for gene silencing in
human macrophages. Altering gene expression can be useful to study the contribution of the
gene to macrophage function or even expression of other genes. Curr. Protoc. Immunol. 96:
14.28. 1‐14.28. 23.© 2012 by John Wiley & Sons, Inc.
includes protocols for human macrophage preparation, RNA extraction, real‐time PCR
analysis, and microarray analysis. The unit also includes a protocol for gene silencing in
human macrophages. Altering gene expression can be useful to study the contribution of the
gene to macrophage function or even expression of other genes. Curr. Protoc. Immunol. 96:
14.28. 1‐14.28. 23.© 2012 by John Wiley & Sons, Inc.
Abstract
This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real‐time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes. Curr. Protoc. Immunol. 96:14.28.1‐14.28.23. © 2012 by John Wiley & Sons, Inc.
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