Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin

EJ Choi, Z Xia, DR Storm - Biochemistry, 1992 - ACS Publications
EJ Choi, Z Xia, DR Storm
Biochemistry, 1992ACS Publications
Department of Pharmacology, University of Washington, Seattle, Washington 98195
Received February 13, 1992; Revised Manuscript Received April 15, 1992 abstract:
Characterization of adenylyl cyclases has been facilitated bythe isolation of cDNA clones for
distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I
adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by
calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is …
Department of Pharmacology, University of Washington, Seattle, Washington 98195 Received February 13, 1992; Revised Manuscript Received April 15, 1992 abstract: Characterization of adenylyl cyclases has been facilitated bythe isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is regulated by stimulatory G proteins. The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. It was, however, stimulated by Ca2+ through calmodulin when theenzyme was concomitantly activated by either GppNHp or forskolin. The concentrations of free Ca2+ for half-maximal stimulation of type I and type III adenylyl cyclases were 0.05 and 5.0/iM Ca2+, respectively. These data suggest that the type III adenylyl cyclase is stimulated by Ca2+ when the enzyme is activated by G-protein-coupled receptors and that increases in free Ca2+ accompanying receptor activation may amplify the primary cyclic AMP signal.
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