Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain

GA Bishop, NF Berbari, J Lewis… - Journal of Comparative …, 2007 - Wiley Online Library
Journal of Comparative Neurology, 2007Wiley Online Library
Solitary primary cilia project from nearly every cell type in the human body. These organelles
are considered to have important sensory and signaling functions. Although primary cilia
have been detected throughout the mammalian brain, their functions are unknown. The
study of primary cilia in the brain is constrained by the scarcity of specific markers for these
organelles. We previously demonstrated that type III adenylyl cyclase (ACIII) is a marker for
primary cilia on neonatal hippocampal neurons in vivo and in vitro. We further showed that …
Abstract
Solitary primary cilia project from nearly every cell type in the human body. These organelles are considered to have important sensory and signaling functions. Although primary cilia have been detected throughout the mammalian brain, their functions are unknown. The study of primary cilia in the brain is constrained by the scarcity of specific markers for these organelles. We previously demonstrated that type III adenylyl cyclase (ACIII) is a marker for primary cilia on neonatal hippocampal neurons in vivo and in vitro. We further showed that ACIII localizes to cilia on cultured glial cells. Here, we report that ACIII is a marker for primary cilia throughout many regions of the adult mouse brain. Furthermore, we report that ACIII localizes to primary cilia on choroid plexus cells and some astrocytes in the brain, which to our knowledge is the first report of a marker for visualizing cilia on glia in vivo. Overall, our data indicate that ACIII is a prominent marker of primary cilia in the brain and will provide an important tool to facilitate further investigations into the functions of these organelles. J. Comp. Neurol. 505:562–571, 2007. © 2007 Wiley‐Liss, Inc.
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